Development of ascorbate transporters in brain cortical capillary endothelial cells in culture
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چکیده
منابع مشابه
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15 صفحه اولHyperosmotic arabinose solutions open the tight junctions between brain capillary endothelial cells in tissue culture.
Tight junctions between bovine brain capillary endothelial cells in primary cell culture are impermeable to horseradish peroxidase (HRP) after 5 min of incubation. However, following 1-5 min of exposure to 1.6 M arabinose, HRP penetrates the extracellular space between successive tight junctions. Endothelial cells in control cultures contain a small number of cytoplasmic pits and vesicles conta...
متن کاملDevelopment and characterisation of a rat brain capillary endothelial culture: towards an in vitro blood-brain barrier.
Primary culture of rat brain endothelial cells is described, based on the method of C. C. W. Hughes and P. L. Lantos. The cells have been characterised using morphological and immunocytochemical techniques, and systematic studies undertaken to determine the optimal culture medium and conditions required to grow the cells at high purity on a variety of substrata. The endothelial cells have a spi...
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The smooth muscle of the abdominal region of the chicken aorta derives from locally recruited mesenchyme (mesenchymal smooth muscle), whereas that of the thoracic region derives from the neural crest (ectomesenchymal smooth muscle). We hypothesized that this smooth muscle heterogeneity might affect important enzymatic functions of the vessel wall. Therefore, we measured angiotensin converting e...
متن کاملNa + - K + - CI - Cotransporter of Brain Capillary Endothelial Cells
Cultured rat brain capillary endothelial cells expressed a large BsRb+ uptake component that was dependent on external Na+ and C1and that was inhibited by loop diuretics with unusual pharmacological properties: benzmetanide (IC, = 1-5 p ~ ) = bumetanide (ICso = 1-5 p ~ ) > piretanide (ICso = 3-16 p ~ ) = furosemide (ICso = 7-11 p ~ ) . It was activated %fold by endothelin-1 (EC, = 1 MI) and end...
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ژورنال
عنوان ژورنال: Brain Research
سال: 2008
ISSN: 0006-8993
DOI: 10.1016/j.brainres.2008.02.102